Volume 22, No 9, Sep 2012

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 22 Issue 9, September 2012: 1390-1401

ORIGINAL ARTICLES

Cep57, a NEDD1-binding pericentriolar material component, is essential for spindle pole integrity

Qixi Wu^1,*, Runsheng He^1,*, Haining Zhou¹, Albert CH Yu², Bo Zhang¹, Junlin Teng¹ and Jianguo Chen^1,3

¹The State Key Laboratory of Biomembrane and Membrane Bioengineering and The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China
²Department of Neurobiology, Neuroscience Research Institute, School of Basic Medical Sciences, Peking University, Beijing 100191, China
³The Center for Theoretical Biology, Peking University, Beijing 100871, China Correspondence: Junlin Teng, +86-10-62755786;(junlinteng@pku.edu.cn; chenjg@pku.edu.cn)

Formation of a bipolar spindle is indispensable for faithful chromosome segregation and cell division. Spindle integrity is largely dependent on the centrosome and the microtubule network. Centrosome protein Cep57 can bundle microtubules in mammalian cells. Its related protein (Cep57R) in Xenopus was characterized as a stabilization factor for microtubule-kinetochore attachment. Here we show that Cep57 is a pericentriolar material (PCM) component. Its interaction with NEDD1 is necessary for the centrosome localization of Cep57. Depletion of Cep57 leads to unaligned chromosomes and a multipolar spindle, which is induced by PCM fragmentation. In the absence of Cep57, centrosome microtubule array assembly activity is weakened, and the spindle length and microtubule density decrease. As a spindle microtubule-binding protein, Cep57 is also responsible for the proper organization of the spindle microtubule and localization of spindle pole focusing proteins. Collectively, these results suggest that Cep57, as a NEDD1-binding centrosome component, could function as a spindle pole- and microtubule-stabilizing factor for establishing robust spindle architecture.

Cell Research (2012) 22:1390-1401. doi:10.1038/cr.2012.61; published online 17 April 2012

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