Volume 21, No 7, Jul 2011

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 21 Issue 7, July 2011: 1088-1101

ORIGINAL ARTICLES

Regulation of cellular senescence by the essential caveolar component PTRF/Cavin-1

Lin Bai, Xiaoli Deng Juanjuan Li, Miao Wang, Qian Li, Wei An, Deli A and Yu-Sheng Cong

Key Lab of Cell Proliferation and Regulation Biology of the Ministry of Education, Institute of Cell Biology, Beijing Normal University, 19 Xin Jie Kou Wai Avenue, Beijing 100875, China Correspondence: Yu-Sheng Cong,(yscong@bnu.edu.cn)

Polymerase I and transcript release factor (PTRF, also known as Cavin-1) is an essential component in the biogenesis and function of caveolae. Here, we show that PTRF expression is increased in senescent human fibroblasts. Importantly, overexpression of PTRF induced features characteristic of cellular senescence, whereas reduced PTRF expression extended the cellular replicative lifespan. Interestingly, we found that PTRF localized primarily to the nuclei of young and quiescent WI-38 human fibroblasts, but translocated to the cytosol and plasma membrane during cellular senescence. Furthermore, electron microscopic analysis demonstrated an increased number of caveolar structures in senescent and PTRF-transfected WI-38 cells. Our data suggest that the role of PTRF in cellular senescence is dependent on its targeting to caveolae and its interaction with caveolin-1, which appeared to be regulated by the phosphorylation of PTRF. Taken together, our findings identify PTRF as a novel regulator of cellular senescence that acts through the p53/p21 and caveolar pathways.

Cell Research (2011) 21:1088-1101. doi:10.1038/cr.2011.56; published online 29 March 2011

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