Volume 20, No 3, Mar 2010
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 20 Issue 3, March 2010: 288-298
ORIGINAL ARTICLES
Bone marrow-derived pancreatic stellate cells in rats
Gisela Sparmann1,*, Marie-Luise Kruse2,*, Nicole Hofmeister-Mielke1,3, Dirk Koczan4, Robert Jaster1, Stefan Liebe1, Daniel Wolff1,3 and Jörg Emmrich1
1Division of Gastroenterology, Department of Internal Medicine, University of Rostock, 18057 Rostock, Germany
2Laboratory for Molecular Gastroenterology and Hepatology, Department of General Internal Medicine, UKSH Campus Kiel, 24105 Kiel, Germany
3Department of Hematology and Internal Oncology, University Hospital Regensburg, 93503 Regensburg, Germany
4Institute of Immunology, University of Rostock, 18057 Rostock, Germany
Correspondence: Gisela Sparmann,(gisela.sparmann@med.uni-rostock.de)
Origin and fate of pancreatic stellate cells (PSCs) before, during and after pancreatic injury are a matter of debate. The crucial role of PSCs in the pathogenesis of pancreatic fibrosis is generally accepted. However, the turnover of the cells remains obscure. The present study addressed the issue of a potential bone marrow (BM) origin of PSCs. We used a model of stable hematopoietic chimerism by grafting enhanced green fluorescence protein (eGFP)-expressing BM cells after irradiation of acceptor rats. Chimerism was detected by FACS analysis of eGFP-positive cells in the peripheral blood. Dibutyltin dichloride (DBTC) was used to induce acute pancreatic inflammation with subsequent recovery over 4 weeks. Investigations have been focused on isolated cells to detect the resting PSC population. The incidence of eGFP-positive PSC obtained from the pancreas of chimeric rats was approximately 7% in healthy pancreatic tissue and increased significantly to a mean of 18% in the restored pancreas 4 weeks after DBTC-induced acute inflammation. Our results suggest that BM-derived progenitor cells represent a source of renewable stellate cells in the pancreas. Increased numbers of resting PSCs after regeneration point toward enhanced recruitment of BM-derived cells to the pancreas and/or re-acquisition of a quiescent state after inflammation-induced activation.
Cell Research (2010) 20:288-298. doi: 10.1038/cr.2010.10; published online 26 January 2010
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