Volume 20, No 2, Feb 2010
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 20 Issue 2, February 2010: 154-165
ORIGINAL ARTICLES
Dual-specificity histone demethylase KIAA1718 (KDM7A) regulates neural differentiation through FGF4
Chengyang Huang1,*, Yang Xiang2,*, Yanru Wang2, Xia Li2, Longyong Xu2, Ziqi Zhu2, Ting Zhang1, Qingqing Zhu1, Kejing Zhang1, Naihe Jing1 and Charlie Degui Chen2
1Laboratory of Molecular Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
2State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
Correspondence: Charlie Degui Chen, Naihe Jing,(cdchen@sibs.ac.cn; njing@sibs.ac.cn)
Dimethylations of histone H3 lysine 9 and lysine 27 are important epigenetic marks associated with transcription repression. Here, we identified KIAA1718 (KDM7A) as a novel histone demethylase specific for these two repressing marks. Using mouse embryonic stem cells, we demonstrated that KIAA1718 expression increased at the early phase of neural differentiation. Knockdown of the gene blocked neural differentiation and the effect was rescued by the wild-type human gene, and not by a catalytically inactive mutant. In addition, overexpression of KIAA1718 accelerated neural differentiation. We provide the evidence that the pro-neural differentiation effect of KDM7A is mediated through direct transcriptional activation of FGF4, a signal molecule implicated in neural differentiation. Thus, our study identified a dual-specificity histone demethylase that regulates neural differentiation through FGF4.
Cell Research (2010) 20:154-165. doi: 10.1038/cr.2010.5; published online 19 January 2010
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