Volume 19, No 12, Dec 2009

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 19 Issue 12, December 2009: 1334-1349

ORIGINAL ARTICLES

The retromer component SNX6 interacts with dynactin p150^Glued and mediates endosome-to-TGN transport

Zhi Hong^1,2,*, Yanrui Yang^1,*, Cheng Zhang¹, Yang Niu^1,2, Ke Li^1,2, Xi Zhao^1,2 and Jia-Jia Liu¹

¹Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

²Graduate School, Chinese Academy of Sciences, Beijing 100039, China Correspondence: Jia-Jia Liu,(jjliu@genetics.ac.cn)

The retromer is a protein complex that mediates retrograde transport of transmembrane cargoes from endosomes to the trans-Golgi network (TGN). It is comprised of a cargo-selection subcomplex of Vps26, Vps29 and Vps35 and a membrane-binding coat subcomplex of sorting nexins (SNXs). Previous studies identified SNX1/2 as one of the components of the SNX subcomplex, and SNX5/6 as candidates for the second SNX. How the retromer-associated cargoes are recognized and transported by molecular motors are largely unknown. In this study, we found that one of SNX1/2's dimerization partners, SNX6, interacts with the p150^Glued subunit of the dynein/dynactin motor complex. We present evidence that SNX6 is a component of the retromer, and that recruitment of the motor complex to the membrane-associated retromer requires the SNX6-p150^Glued interaction. Disruption of the SNX6-p150^Glued interaction causes failure in formation and detachment of the tubulovesicular sorting structures from endosomes and results in block of CI-MPR retrieval from endosomes to the TGN. These observations indicate that in addition to SNX1/2, SNX6 in association with the dynein/dynactin complex drives the formation and movement of tubular retrograde intermediates.

Cell Research (2009) 19:1334-1349. doi: 10.1038/cr.2009.130; published online 24 November 2009

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