Volume 19, No 12, Dec 2009

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 19 Issue 12, December 2009: 1388-1400

ORIGINAL ARTICLES

P68 RNA helicase is a nucleocytoplasmic shuttling protein

Haizhen Wang^1,*, Xueliang Gao^1,*, Yun Huang², Jenny Yang² and Zhi-Ren Liu¹

¹Departments of Biology, Georgia State University, Atlanta, GA 30303, USA

²Departments of Chemistry, Georgia State University, Atlanta, GA 30303, USA Correspondence: Zhi-Ren Liu,(biozrl@langate.gsu.edu)

P68 RNA helicase is a prototypical DEAD box RNA helicase. The protein plays a very important role in early organ development and maturation. Consistent with the function of the protein in transcriptional regulation and pre-mRNA splicing, p68 was found to predominately localize in the cell nucleus. However, recent experiments demonstrate a transient cytoplasmic localization of the protein. We report here that p68 shuttles between the nucleus and the cytoplasm. The nucleocytoplasmic shuttling of p68 is mediated by two nuclear localization signal and two nuclear exporting signal sequence elements. Our experiments reveal that p68 shuttles via a classical RanGTPase-dependent pathway.

Cell Research (2009) 19:1388-1400. doi: 10.1038/cr.2009.113; published online 29 September 2009

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