Volume 19, No 11, Nov 2009
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 19 Issue 11, November 2009: 1291-1304
ORIGINAL ARTICLES
An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis
Rui-Ling Mu1,*, Yang-Rong Cao1,*, Yun-Feng Liu1,*, Gang Lei1, Hong-Feng Zou1, Yong Liao1, Hui-Wen Wang1, Wan-Ke Zhang1, Biao Ma1, Ji-Zhou Du2, Ming Yuan2, Jin-Song Zhang1 and Shou-Yi Chen1
1Plant Gene Research Center, National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2National Key Lab of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
Correspondence: Jin-Song Zhang, Shou-Yi Chen,(jszhang@genetics.ac.cn; sychen@genetics.ac.cn )
MYB proteins play important roles in eukaryotic organisms. In plants, the R1R2R3-type MYB proteins function in cell cycle control. However, whether the R2R3-type MYB protein is also involved in the cell division process remains unknown. Here, we report that an R2R3-type transcription factor gene, AtMYB59, is involved in the regulation of cell cycle progression and root growth. The AtMYB59 protein is localized in the nuclei of onion epidermal cells and has transactivation activity. Expression of AtMYB59 in yeast cells suppresses cell proliferation, and the transformants have more nuclei and higher aneuploid DNA content with longer cells. Mutation in the conserved domain of AtMYB59 abolishes its effects on yeast cell growth. In synchronized Arabidopsis cell suspensions, the AtMYB59 gene is specifically expressed in the S phase during cell cycle progression. Expression and promoter-GUS analysis reveals that the AtMYB59 gene is abundantly expressed in roots. Transgenic plants overexpressing AtMYB59 have shorter roots compared with wild-type plants (Arabidopsis accession Col-0), and around half of the mitotic cells in root tips are at metaphase. Conversely, the null mutant myb59-1 has longer roots and fewer mitotic cells at metaphase than Col, suggesting that AtMYB59 may inhibit root growth by extending the metaphase of mitotic cells. AtMYB59 regulates many downstream genes, including the CYCB1;1 gene, probably through binding to MYB-responsive elements. These results support a role for AtMYB59 in cell cycle regulation and plant root growth.
Cell Research (2009) 19:1291-1304. doi: 10.1038/cr.2009.83; published online 7 July 2009
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