Volume 19, No 10, Oct 2009
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 19 Issue 10, October 2009: 1205-1216
ORIGINAL ARTICLES
A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Xiaozhen Huang1, Xiaoyan Zhang1 and Shuhua Yang1,2
1State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
2National Plant Gene Research Center, Beijing 100193, China
Correspondence: Shuhua Yang,(yangshuhua@cau.edu.cn )
To understand the molecular mechanisms underlying chloroplast development, we isolated and characterized the albino mutant emb1303-1 in Arabidopsis. The mutant displayed a severe dwarf phenotype with small albino rosette leaves and short roots on a synthetic medium containing sucrose. It is pigment-deficient and seedling lethal when grown in soil. Embryo development was delayed in the mutant, although seed germination was not significantly impaired. The plastids of emb1303-1 were arrested in early developmental stages without the classical stack of thylakoid membrane. Genetic and molecular analyses uncovered that the EMB1303 gene encodes a novel chloroplast-localized protein. Microarray and RT-PCR analyses revealed that a number of nuclear- and plastid-encoded genes involved in photosynthesis and chloroplast biogenesis were substantially downregulated in the mutant. Moreover, the accumulation of several major chloroplast proteins was severely compromised in emb1303-1. These results suggest that EMB1303 is essential for chloroplast development.
Cell Research (2009) 19:1205-1216. doi: 10.1038/cr.2009.84; published online 7 July 2009
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