Volume 19, No 6, Jun 2009
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 19 Issue 6, June 2009: 747-757
ORIGINAL ARTICLES
High levels of dRYBP induce apoptosis in Drosophila imaginal cells through the activation of reaper and the requirement of trithorax, dredd and dFADD
Inma González and Ana Busturia
Centro de Biología Molecular "Severo Ochoa" CSIC-UAM, c) Nicolás Cabrera 1, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain
Correspondence: Ana Busturia,(abusturia@cbm.uam.es )
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Drosophila RYBP (
dRYBP;
Ring1 and
YY1
Binding
Protein) is a Polycomb and trithorax interacting protein, whose homologous RYBP/DEDAF mammalian counterparts exhibit tumor cell-specific killing activity. Here we show that although endogenous dRYBP is not involved in developmental apoptosis, high levels of exogenous dRYBP induce apoptosis in all the imaginal discs of the fly, indicating that dRYBP apoptotic activity is not specific to tumor cells. We also show that dRYBP-induced apoptosis is inhibited by high levels of either p35 or DIAP1 (
Drosophila
Inhibitor of
Apoptosis
Protein 1), and requires the function of the pro-apoptotic REAPER, HID and GRIM proteins, the apical caspase DREDD, the adaptor dFADD protein as well as TRITHORAX (TRX), an epigenetic transcriptional regulator. Furthermore, we demonstrate that overexpression of TRX also induces apoptosis in the imaginal discs. Finally, we show that the expression of
reaper-lacZ is upregulated both upon dRYBP-induced apoptosis and upon TRX-induced apoptosis in imaginal discs and that the
reaper gene is a direct target of dRYBP in
Drosophila embryos. Our results indicate that dRYBP triggers in a receptor-mediated apoptotic pathway that also includes TRX-dependent epigenetic regulation of gene expression.
Cell Research (2009) 19:747-757. doi: 10.1038/cr.2009.29; published online 3 March 2009
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