Volume 18, No 9, Sep 2008

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 18 Issue 9, September 2008: 949-960

ORIGINAL ARTICLES

Heterotrimeric G-protein is involved in phytochrome A-mediated cell death of Arabidopsis hypocotyls

Qing Wei, Wenbin Zhou, Guangzhen Hu, Jiamian Wei, Hongquan Yang and Jirong Huang

National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China Correspondence: Jirong Huang(huangjr@sibs.ac.cn)

The heterotrimeric guanine nucleotide-binding protein (G-protein) has been demonstrated to mediate various signaling pathways in plants. However, its role in phytochrome A (phyA) signaling remains elusive. In this study, we discover a new phyA-mediated phenotype designated far-red irradiation (FR) preconditioned cell death, which occurs only in the hypocotyls of FR-grown seedlings following exposure to white light (WL). The cell death is mitigated in the Gα mutant gpa1 but aggravated in the Gβ mutant agb1 in comparison with the wild type (WT), indicative of antagonistic roles of GPA1 and AGB1 in the phyA-mediated cell-death pathway. Further investigation indicates that FR-induced accumulation of nonphotoconvertible protochlorophyllide (Pchlide⁶³³), which generates reactive oxygen species (ROS) on exposure to WL, is required for FR-preconditioned cell death. Moreover, ROS is mainly detected in chloroplasts using the fluorescent probe. Interestingly, the application of H₂O₂ to dark-grown seedlings results in a phenotype similar to FR-preconditioned cell death. This reveals that ROS is a critical mediator for the cell death. In addition, we observe that agb1 is more sensitive to H₂O₂ than WT seedlings, indicating that the G-protein may also modify the sensitivity of the seedlings to ROS stress. Taking these results together, we infer that the G-protein may be involved in the phyA signaling pathway to regulate FR-preconditioned cell death of Arabidopsis hypocotyls. A possible mechanism underlying the involvement of the G-protein in phyA signaling is discussed in this study.

Cell Research (2008) 18:949-960. doi: 10.1038/cr.2008.271; published online 12 August 2008

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