Volume 18, No 6, Jun 2008
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 18 Issue 6, June 2008: 641-648
ORIGINAL ARTICLES
Differential remodeling of a T-cell transcriptome following CD8- versus CD3-induced signaling
S Hussain I Abidi1,2, Tao Dong1, Mai T Vuong1, Vattipally B Sreenu1, Sarah L Rowland-Jones1, Edward J Evans1 and Simon J Davis1
1Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, The University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK;
2Present address: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Correspondence: Edward J Evans Simon J Davis,(edward.evans@ndm.ox.ac.uk simon.davis@ndm.ox.ac.uk)
CD8 engagement with class I major histocompatibility antigens greatly enhances T-cell activation, but it is not clear how this is achieved. We address the question of whether or not the antibody-mediated ligation of CD8 alone induces transcriptional remodeling in a T-cell clone, using serial analysis of gene expression. Even though it fails to induce overt phenotypic changes, we find that CD8 ligation profoundly alters transcription in the T-cell clone, at a scale comparable to that induced by antibody-mediated ligation of CD3. The character of the resulting changes is distinct, however, with the net effect of CD8 ligation being substantially inhibitory. We speculate that ligating CD8 induces weak, T-cell receptor (TCR)-mediated inhibitory signals reminiscent of the effects of TCR antagonists. Our results imply that CD8 ligation alone is incapable of activating the T-cell clone because it fails to fully induce NFAT-dependent transcription.
Cell Research (2008) 18:641-648. doi: 10.1038/cr.2008.56; published online 13 May 2008
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