Volume 18, No 4, Apr 2008
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 18 Issue 4, April 2008: 479-490
ORIGINAL ARTICLES
Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay
Zhi-hui Huang1,2, Ying Wang1, Li Cao2, Zhi-da Su2, Yan-ling Zhu2, Yi-zhang Chen1,2, Xiao-bing Yuan3 and Cheng He1,2
1Department of Neurobiology, Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
2Department of Neurobiology, Second Military Medical University, Shanghai 200433, China
3Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Correspondence: Cheng He(chenghe@online.sh.cn )
Olfactory ensheathing cells (OECs) are a unique type of glial cells that have axonal growth-promoting properties. OEC transplantation has emerged as a promising experimental therapy of axonal injuries and demyelinating diseases. However, some fundamental cellular properties of OECs remain unclear. In this study, we found that the distinct OEC subpopulations exhibited different migratory properties based on time-lapse imaging of single isolated cells, possibly due to their different cytoskeletal organizations. Moreover, OEC subpopulations displayed different attractive migratory responses to a gradient of lysophosphatidic acid (LPA) in single-cell migration assays. Finally, we found that OEC subpopulations transformed into each other spontaneously. Together, these results demonstrate, for the first time to our knowledge, that distinct OEC subpopulations display different migratory properties in vitro and provide new evidence to support the notion of OECs as a single cell type with malleable functional phenotypes.
Cell Research (2008) 18:479-490. doi: 10.1038/cr.2008.38; published online 18 March 2008
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