Volume 18, No 2, Feb 2008

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 18 Issue 2, February 2008: 238-247

REVIEWS

Intracellular protease activation in apoptosis and cell-mediated cytotoxicity characterized by cell-permeable fluorogenic protease substrates

Beverly Z Packard1 and Akira Komoriya

OncoImmunin Inc5., 207A Perry Parkway, Suite 6, Gaithersburg, MD 20877, USA Correspondence: Beverly Z Packard(BPackard@PhiPhiLux.com )

Over the past decade the importance of signaling from reporter molecules inside live cells and tissues has been clearly established. Biochemical events related to inflammation, tumor metastasis and proliferation, and viral infectivity and replication are examples of processes being further defined as more molecular tools for live cell measurements become available. Moreover, in addition to quantitating parameters related to physiologic processes, real-time imaging of molecular interactions that compose basic cellular activities are providing insights into understanding disease mechanisms as well as extending clinical efficacy of therapeutic regimens. In this review the use of highly cell-permeable fluorogenic substrates that report protease activities inside live cells is described; applications to defining the molecular events of two cellular processes, i.e., apoptosis and cell-mediated cytotoxicity, are then illustrated.

Cell Research (2008) 18:238-247. doi: 10.1038/cr.2008.17; published online 29 January 2008

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