Volume 17, No 8, Aug 2007
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 17 Issue 8, August 2007: 713-721
ORIGINAL ARTICLES
OsRRM, a Spen-like rice gene expressed specifically in the endosperm
Shi-Yan Chen1,2, Zong-Yang Wang1 and Xiu-Ling Cai1
1National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
2Graduate School of the Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100039, China
Correspondence: Xiu-Ling Cai(xlcai@sippe.ac.cn)
We used the promoter trap technique to identify a rice plant, named 107#, in which the β-glucuronidase (GUS) reporter gene was expressed specifically in the endosperm. A single copy of the T-DNA was inserted into the plant genome, and a candidate gene OsRRM was identified by the insertion. The OsRRM promoter directed GUS expression specifically in rice endosperm, analogous to the GUS expression pattern observed in 107#. OsRRM is a single-copy gene in rice and encodes a nuclear protein containing 1 005 amino-acid residues with two RNA recognition motifs and one Spen paralog and ortholog C-terminal domain. Western blot analysis confirmed that the OsRRM protein was specifically expressed in rice endosperm. Ectopic expression of OsRRM in transgenic plants led to abnormalities, such as short stature, retarded growth and low fructification rates. Our data, in conjunction with the reported function of Spen genes, implicated OsRRM in the regulation of cell development in rice endosperm.
Cell Research (2007) 17:713-721. doi: 10.1038/cr.2007.43; published online 8 May 2007
FULL TEXT | PDF
Browse 1767
