Volume 17, No 7, Jul 2007

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 17 Issue 7, July 2007: 619-626

ORIGINAL ARTICLES

Interactions of primary neuroepithelial progenitor and brain endothelial cells: distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact

Miguel A Gama Sosa^1,3, Rita De Gasperi^1,3, Anne B Rocher², Gissel M Perez^1,3, Keila Simons^1,3, Daniel E Cruz^1,3, Patrick R Hof² and Gregory A Elder^1,3

¹Department of Psychiatry, Mount Sinai School of Medicine of New York University, New York, NY;
²Department of Neuroscience, Mount Sinai School of Medicine of New York University, New York, NY;
³Research and Development Service, James J. Peters Department of Veterans Affairs Medical Center, 130 West Kingsbridge Road, Bronx, New York 10468, USA Correspondence: Miguel A Gama Sosa(miguel.gama-sosa@mssm.edu)

Neurovascular interactions are crucial for the normal development of the central nervous system. To study such interactions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelial cell fractions from embryonic mouse brains. Depending on the culture conditions endothelial cells were found to favor maintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differentiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellular interactions needed for the proper development of the brain.

Cell Research (2007) 17:619-626. doi: 10.1038/cr.2007.53; published online 26 June 2007

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