Volume 17, No 7, Jul 2007
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 17 Issue 7, July 2007: 638-649
ORIGINAL ARTICLES
The role of water channel proteins and nitric oxide signaling in rice seed germination
Hong-Yan Liu1, Xin Yu1, Da-Yong Cui1, Mei-Hao Sun1, Wei-Ning Sun1, Zhang-Cheng Tang1, Sang-Soo Kwak2 and Wei-Ai Su1
1Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
2Korea Research Institute of Bioscience and Biotechnology, Oun-dong 52, Yusong, Taejon 305-333, South Korea
Correspondence: Wei-Ai Su(zstressc@online.sh.cn)
Previous studies have demonstrated the possible role of several aquaporins in seed germination. But systematic investigation of the role of aquaporin family members in this process is lacking. Here, the developmental regulation of plasma membrane intrinsic protein (PIP) expression throughout germination and post-germination processes in rice embryos was analyzed. The expression patterns of the PIPs suggest these aquaporins play different roles in seed germination and seedling growth. Partial silencing of the water channel genes, OsPIP1;1 and OsPIP1;3, reduced seed germination while over-expression of OsPIP1;3 promoted seed germination under water-stress conditions. Moreover, spatial expression analysis indicates that OsPIP1;3 is expressed predominantly in embryo during seed germination. Our data also revealed that the nitric oxide (NO) donors, sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO), promoted seed germination; furthermore, the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, inhibited germination and reduced the stimulative effects of SNP and GSNO on rice germination. Exogenous NO stimulated the transcription of OsPIP1;1, OsPIP1;2, OsPIP1;3 and OsPIP2;8 in germinating seeds. These results suggest that water channels play an important role in seed germination, acting, at least partly, in response to the NO signaling pathway.
Cell Research (2007) 17:638-649. doi: 10.1038/cr.2007.34; published online 24 April 2007
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