Volume 17, No 4, Apr 2007
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 17 Issue 4, April 2007: 374-383
ORIGINAL ARTICLES
C/EBPαp30 plays transcriptional regulatory roles distinct from C/EBPαp42
Chunxi Wang1, Xiaotao Chen1, Yanping Wang1, Jialei Gong1, Gengxi Hu1
1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
Correspondence: Gengxi Hu(hgxgene@sunm.shcnc.ac.cn)
CCAAT/enhancer binding protein α (C/EBPα) is a transcriptional regulatory factor that inhibits cell proliferation, and
alternative translational initiation produces two polypeptides, C/EBPαp30 and C/EBPαp42. By expression profiling,
it was revealed that C/EBPαp30 specifically inhibited a unique set of genes, including MPP11, p84N5 and SMYD2,
which were not affected by C/EBPαp42 in both QSG-7701 hepatocyte cell line and QGY-7703 hepatoma cells. Semiquantitative
RT-PCR analysis independently confirmed these results. Chromatin immunoprecipitation assay showed that
C/EBPαp30 bound to the promoters of these genes more strongly than C/EBPαp42. In clinical hepatoma samples in
which C/EBPα was downregulated, all three genes specifically inhibited by C/EBPαp30 were upregulated. However,
repression of MPP11, p84N5 and SMYD2 genes might not be directly involved in C/EBPαp30-mediated growth inhibition.
Our data suggest that C/EBPαp30 regulates a unique set of target genes and is more than a dominant-negative
regulator of C/EBPαp42.
Cell Research (2007) 17: 374-383 doi: 10.1038/sj.cr.7310121; published online 23 January 2007
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