Volume 17, No 3, Mar 2007
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 17 Issue 3, March 2007: 249-263
ORIGINAL ARTICLES
Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development
Genji Qin1, Zhiqiang Ma1, Li Zhang1, Shufan Xing1, Xianhui Hou1, Jie Deng1, Jingjing Liu1, Zhangliang Chen1,2, Li-Jia Qu1,2 and Hongya Gu1,2
1National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular
Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China; 2The National Plant Gene
Research Center (Beijing), Beijing 100101, China
Correspondence: Hongya Gu(guhy@pku.edu.cn )
Pollen germination on the surface of compatible stigmatic tissues is an essential step for plant fertilization. Here we report that the Arabidopsis mutant bcl1 is male sterile as a result of the failure of pollen germination. We show that the bcl1 mutant allele cannot be transmitted by male gametophytes and no homozygous bcl1 mutants were obtained. Analysis of pollen developmental stages indicates that the bcl1 mutation affects pollen germination but not pollen maturation. Molecular analysis demonstrates that the failure of pollen germination was caused by the disruption of AtBECLIN 1. AtBECLIN 1 is expressed predominantly in mature pollen and encodes a protein with significant homology to Beclin1/Atg6/Vps30 required for the processes of autophagy and vacuolar protein sorting (VPS) in yeast. We also show that AtBECLIN 1 is required for normal plant development, and that genes related to autophagy, VPS and the glycosylphosphatidylinositol anchor system, were affected by the deficiency of AtBECLIN 1.
Cell Research (2007) 17: 249-263. doi: 10.1038/cr.2007.7; published online 6 March 2007
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