Volume 16, No 10, Oct 2006

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 16 Issue 10, October 2006: 818-829

ORIGINAL ARTICLES

Sinorhizobium meliloti nifA mutant induces different gene expression profile from wild type in Alfalfa nodules

Zi Ying Gong, Zhi Shui He, Jia Bi Zhu, Guan Qiao Yu, Hua Song Zou

National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China Correspondence: Hua Song Zou(hszhou@sibs.ac.cn.)

Several studies have demonstrated that the Rhizobium nifA gene is an activator of nitrogen fixation acting in nodule bacteria. To understand the effects of the Sinorhizobium meliloti nifA gene on Alfalfa, the cDNA-AFLP technique was employed to study the changes in gene expression in nifA mutant nodules. Among the approximately 3,000 transcriptderived fragments, 37 had differential expression levels. These expression levels were subsequently confirmed by reverse Northern blot and RT-polymerase chain reaction. Sequence analyses revealed that 21 cDNA fragments corresponded to genes involved in signal communication, protein degradation, nutrient metabolism, cell growth and development.

Cell Research (2006) 16:818-829. doi:10.1038/sj.cr.7310096; published online 26 September 2006

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