Volume 16, No 9, Sep 2006
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 16 Issue 9, September 2006: 759-770
ORIGINAL ARTICLES
A new interaction between Abi-1 and βPIX involved in PDGF-activated actin cytoskeleton reorganisation
Fanny Campa, Nikolaus Machuy, Alexander Klein, Thomas Rude
1Max Planck Institute for Infection Biology, Department of Molecular Biology, Campus Charité Mitte, Charitéplatz 1, Berlin 10 117,Germany;
2Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
Correspondence: Thomas Rudel(rudel@mpiib-berlin.mpg.de)
Members of the Rho family of GTPases are key regulators of the actin cytoskeleton. In particular, activated Rac1
stimulates membrane dorsal ruffle formation in response to platelet-derived growth factor (PDGF). Abl-interactor (Abi)-
1 and βPIX, a guanine nucleotide exchange factor for Rac1, localise at these Rac1-induced actin structures and play
important roles in the induction of membrane dorsal ruffling in response to PDGF in fibroblasts. Here, we demonstrate
a novel interaction between Abi-1 and βPIX using the yeast two-hybrid system, in vitro pull-down assays, and in vivo
co-immunoprecipitation experiments. In vitro, the C-terminal fragment of βPIX interacted with Abi-1, while in vivo the
N-terminal fragment of βPIX interacted with Abi-1. The biological function of this interaction was investigated in mouse
fibroblasts in response to PDGF stimulation. Abi-1 and βPIX co-localised in the cytoplasm and to membrane dorsal
ruffles after PDGF treatment. We show that the co-expression of Abi-1 and truncated forms of βPIX in mouse fibroblasts
blocked PDGF-induced membrane dorsal ruffles. Together, these results show that the interaction between Abi-1 and
βPIX is involved in the formation of growth factor-induced membrane dorsal ruffles.
Cell Research (2006) 16:759-770. doi: 10.1038/sj.cr.7310091; published online 29 Aug 2006
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