Volume 16, No 6, Jun 2006

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 16 Issue 6, June 2006: 539-547

ORIGINAL ARTICLES

A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins

Yankun Gao, Mei Jiang, Tao Yang, Jian Ni, Jiangye Chen

¹State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China Correspondence: Jiangye Chen(jychen@sunm.shcnc.ac.cn)

hPFTAIRE1 (PFTK1), a Cdc2-related protein kinase, is highly expressed in human brain. It exhibits cytoplasmic
distribution in Hela cells, although it contains two nuclear localization signals (NLSs) in its N-terminus. To search for its
substrates and regulatory components, we screened a two-hybrid library by using the full-length hPFTAIRE1 as a bait.
Four 14-3-3 isoforms (β, ε, η, τ) were identified interacting with the hPFTAIRE1. We found a putative 14-3-3 binding
consensus motif (RHSSPSS) in the hPFTAIRE1, which overlapped with its second NLS. Deletion of the RHSSPSS motif
or substitution of Ser¹¹⁹ with Ala in the conserved binding motif abolished the specific interaction between the hPFTAIRE1
and the 14-3-3 proteins. The mutant S120A hPFTAIRE1 also showed a weak interaction to the 14-3-3 proteins. The results
suggested that the Ser¹¹⁹ is crucial for the interaction between hPFTAIRE1 and the 14-3-3 proteins. All the hPFTAIRE1
mutants distributed in cytoplasm of Hela cells and human neuroblastoma cells (SH-SY5Y) when fused to the C-terminus
of a green fluorescent protein (GFP), indicating that binding with the 14-3-3 proteins does not contribute to the subcellular
localization of the hPFTAIRE1, although the binding may be involved in its signaling regulation.

Cell Research (2006) 16:539-547. doi:10.1038/sj.cr.7310071; published online 15 June 2006

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