Volume 16, No 1, Jan 2006
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 16 Issue 1, January 2006: 55-69
ORIGINAL ARTICLES
Preassembly and ligand-induced restructuring of the chains of the IFN-γ receptor complex: the roles of Jak kinases, Stat1 and the receptor chains
Christopher D Krause1, Natasha Lavnikova1, Junxia Xie1, Erwen Mei2, Olga V Mirochnitchenko1, Yiwei Jia3, Robin M Hochstrasser2, Sidney Pestka1,4,5
1Department of Molecular Genetics, Microbiology and Immunology, The University of Medicine and Dentistry of New Jersey -Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, NJ 08854, USA;
2Regional Laser Biotechnology Laboratories, Department of Chemistry, The University of Pennsylvania, Philadelphia, PA 19103, USA;
3Olympus America Inc., SEG, Two Corporate Center Drive, Melville, NY 11747-3157, USA; 4Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08903-2681, USA; 5PBL Biomedical Laboratories, 131 Ethel Road West, Suite 6, Piscataway, NJ 08854-5900, USA
Correspondence: Sidney Pestka(pestka@umdnj.edu)
We previously demonstrated using noninvasive technologies that the interferon-gamma (IFN-γ) receptor complex is preassembled [1]. In this report we determined how the receptor complex is preassembled and how the ligand-mediated conformational changes occur. The interaction of Stat1 with IFN-γR1 results in a conformational change localized to IFN-γR1. Jak1 but not Jak2 is required for the two chains of the IFN-γ receptor complex (IFN-γR1 and IFN-γR2) to interact; however, the presence of both Jak1 and Jak2 is required to see any ligand-dependant conformational change. Two IFN-γR2 chains interact through species-specific determinants in their extracellular domains. Finally, these determinants also participate in the interaction of IFN-γR2 with IFN-γR1. These results agree with a detailed model of the IFN-γ receptor that requires the receptor chains to be pre-associated constitutively for the receptor to be active.
Cell Research (2006) 16:55-69. doi:10.1038/sj.cr.7310008; published online 16 January 2006
FULL TEXT | PDF
Browse 1837
