Volume 16, No 1, Jan 2006

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 16 Issue 1, January 2006: 106-112

ORIGINAL ARTICLES

The identification of a new actin-binding region in p57

Chang Zhen Liu¹, Yong Chen¹, Sen Fang Sui¹

¹Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembranes, Tsinghua University, Beijing 100084, China Correspondence: Sen Fang Sui(suisf@mail.tsinghua.edu.cn)

The actin-binding protein p57 is a member of mammalian coronin-like proteins. The roles of this protein in phagocytic processes conceivably depend on its interactions with F-actin. Two regions, p57^1-34and p57^111-204, were previously reported to be actin-binding sites. In this study, we found that the C-terminal region of p57, p57^297-461, also possessed F-actin binding activity. Furthermore, the leucine zipper domain at the C-terminus of p57^297-461 was essential for this actin-binding activity. The F-actin cross-linking assay revealed that the region contained in p57^297-461 was sufficient to cross-link actin filaments. Our results strongly suggested that there was a new actin-binding region at the C-terminus of p57.

Cell Research (2006) 16:106-112 . doi:10.1038/sj.cr.7310014; published online 16 January 2006

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