Volume 15, No 10, Oct 2005

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 15 Issue 10, October 2005: 770-776

ORIGINAL ARTICLES

Systemic delivery of full-length C/EBPb / liposome complex suppresses growth of human colon cancer in nude mice

Li SUN, Bei Bei FU, Ding Gan LIU*

State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China Correspondence: Ding Gan LIU(E-mail:dgliu@sibs.ac.cn)

C/EBPb (CCAAT/enhancer-binding protein b) is an important transcription factor involved in cellular proliferation and differentiation. Overexpression of the full-length C/EBPb protein results in cellular growth arrest and apoptosis. Using a nonviral liposome as carrier, we delivered the full-length C/EBPb expression plasmid, pCN, into nude mice bearing CW-2 human colon cancer tumors via tail vein. Southern blots revealed that the major organs and tumors were transfected. Experimental gene therapy showed that a strong suppression of tumor growth was observed in the pCN-treated mice, and such suppression was due to the overexpression of C/EBPb, leading to the increased apoptosis in tumors of pCN-treated mice. No apparent toxic effects of pCN/liposome complex were observed in the animals. Thus, C/EBPb has tumor suppression effect in vivo and may be used in gene therapy for cancers.

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