Volume 15, No 5, May 2005
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 15 Issue 5, May 2005: 371-378
ORIGINAL ARTICLES
Involvement of regulatory volume decrease in the migration of nasopharyngeal carcinoma cells
Jian Wen MAO1, Li Wei WANG2, 3, Tim JACOB3, Xue Rong SUN2, Hui LI1, Lin Yan ZHU2, Pan LI1, Ping ZHONG2, Si Huai NIE2, Li Xin CHEN1, 3, *
1Laboratory of Cell Biology, 2Laboratory of Physiology, Guangdong Medical College, Zhanjiang 524023, China
3Cardiff School of Biosciences, Cardiff University, Biomedical Sciences Building, Museum Avenue, Cardiff CF10 3US, UK
Correspondence: Li Xin CHEN(CHENL1@cardiff. ac.uk)
The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5'-triphosphate (ATP), 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non-migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relationship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.
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