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Volume 14, No 3, Jun 2004

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 14 Issue 3, June 2004: 217-226

ORIGINAL ARTICLES

Epigenetic modification regulates both expression of tumor-associated genes and cell cycle progressing in human colon cancer cell lines: Colo-320 and SW1116

Jing Yuan FANG, Ying Xuan CHEN, Juan LU, Rong LU, Li YANG, Hong Yin ZHU, Wei Qi GU, Lun Gen LU

Shanghai Institute of Digestive Disease, Renji Hospital Shanghai Second Medical University, Shanghai 200001, China. Correspondence: Jing Yuan FANG(jingyuanfang@yahoo.com)

The aim of this study is to assess the effects of DNA class=SpellE>methylation and histone class=SpellE>acetylation, alone or in combination, on the expression of
several tumor-associated genes and cell cycle progression in two established
human colon cancer cell lines: Colo-320 and SW1116. Treatments with 5-azaUnitName="’" SourceValue="2" HasSpace="False" Negative="True" NumberType="1"
TCSC="0" w:st="on">-2’-deoxycytidine (5-aza-dC) and class=SpellE>trichostatin A, alone or in combination, were applied respectively.
The methylation status of the CDKNUnitName="a" SourceValue="2" HasSpace="False" Negative="False" NumberType="1"
TCSC="0" w:st="on">2A promoter was determined by class=SpellE>methylation-specific PCR, and the acetylated status of the
class=SpellE>histones associated with the p21WAF1 and
CDKNUnitName="a" SourceValue="2" HasSpace="False" Negative="False" NumberType="1"
TCSC="0" w:st="on">2A genes was examined by chromatin class=SpellE>immunoprecipitation. The expression of the CDKNUnitName="a" SourceValue="2" HasSpace="False" Negative="False" NumberType="1"
TCSC="0" w:st="on">2A, p21WAF1, p53, p73,
APC, c-myc, c-class=SpellE>Ki-ras and survivin genes
was detected by real-time RT-PCR and RT-PCR. The cell cycle profile was
established by flow cytometry.


We found that along with the demethylation
of the CDKNNegative="False" NumberType="1" TCSC="0" w:st="on">2A gene
promoter in both cell lines induced by 5-aza-dC alone or in combination
with TSA, the expression of both CDKNHasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">2A
and APC genes increased. The treatment of TSA or sodium butyrate
up-regulated the transcription of p21WAF1 significantly
by inducing the acetylation of histones
H4 and H3, but failed to alter the acetylation
level of CDKNNegative="False" NumberType="1" TCSC="0" w:st="on">2A-associated
histones. No changes in transcription ofstyle='mso-spacerun:yes'>  p53, p73, c-myc,
c-Ki-ras and survivin
genes were observed. In addition, TSA or sodium butyrate was shown to
arrest cells at the G1 phase. However, 5-aza-dC was not able
to affect the cell cycle progression. In conclusion, regulation
by epigenetic modification of the transcription of tumor-associated genes
and the cell cycle progression in both human colon cancer cell lines Colo-320
and SW1116 is gene-specific.


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