Volume 14, No 1, Feb 2004

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 14 Issue 1, February 2004: 81-85

ORIGINAL ARTICLES

The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species

Fei GAO¹, Jing YI¹, Jing Qi YUAN², Gui Ying SHI¹, Xue Ming TANG¹

¹Department of Cell Biology, Shanghai Second Medical University, Shanghai 200025, China.
²Department of Automation, Shanghai Jiaotong University, Shanghai 200031, China. Correspondence: Xue Ming TANG(xmtang@shsmu.edu.cn )

Double staining flow
cytometry was performed using 7-amino actinomycin D and 6-carboxy-2',
7'-dichlorodihydrofluorescein
diacetate, to detect the level fluctuation of reactive oxygen species
(ROS) during the cell cycle of normal NB4 cells. Our results showed that
NB4 cells possessed higher level of ROS in G2/M phase than in G1 and S
phases. Double staining flow cytometry, with TdT mediated dUTP nick end
labeling (Tunel) and propidium iodide (PI), indicated that As₂O₃
(2 μM) could induce apoptosis in NB4 cells prevailingly from G2/M
phase, and this efficacy was enhanced upon co-administration of 2, 3-dimethoxy-1,
4-naphthoquinone (DMNQ) (2.5 μM) which could produce the endogenous
ROS. These results suggested that different ROS level in different cell
cycle phases of NB4 cells might determin the selective induction of G2/M
apoptosis and the cells' susceptibility to apoptosis by As₂O₃.

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