Volume 13, No 5, Oct 2003
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 13 Issue 5, October 2003: 361-367
ORIGINAL ARTICLES
Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand
JIA HUA HU1,*, JIE JIANG1,2,*, YING HUA MA1,**, NA YANG1,***, MAO HU ZHANG1, MIN WU2, JIAN FEI1,3,****, LI HE GUO1,*****
1Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. E-mail: mhzhang@sunm.shcnc.ac.cn
2College of life science, Zhejiang University, Hangzhou 310027, China
3Shanghai Research Center for Biomodel Organism, Shanghai 201203, China
*These authors contributed equally to this work.
**Present Address: Department of Neurology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02115, USA
***Present Address: Department of Chemistry, Auburn University, Auburn, Alabama 36849, USA
Correspondence: Li He GUO(mhzhang@sunm.shcnc.ac.cn)
It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpressing human FasL to investigate whether Fas ligand plays a role in ethanol-induced testicular germ cell apoptosis. Both wild-type (WT) mice and transgenic (TG) mice were treated with acute ethanol (20% v/v) by introperitoneal injection for five times. After ethanol injection, WT mice displayed up-regulation of Fas ligand in the testes, which was shown by FITC-conjugated flow cytometry and western blotting. Moreover, TG mice exhibited significantly more apoptotic germ cells than WT mice did after ethanol injection, which was demonstrated by DNA fragmentation, PI staining flow cytometry and TUNEL staining. In addition, histopathological examination revealed that degenerative changes of epithelial component of the tubules occurred in FasL overexpressing transgenic mice while testicular morphology was normal in wild-type mice after acute ethanol exposure, suggesting FasL expression determines the sensitivity of testes to ethanol in mice. In summary, we provide the direct evidences that Fas ligand mediates the apoptosis of testicular germ cells induced by acute ethanol using FasL transgenic mice.
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