Volume 13, No 3, Jun 2003
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 13 Issue 3, June 2003: 171-177
ORIGINAL ARTICLES
Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis
Sheng Wei ZOU1, Jian Chao ZHANG2, Xiao Dong ZHANG1, Shi Ying MIAO1, Shu Dong ZONG2, Qi SHENG1, Lin Fang WANG1,*
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan 3-tiao, Beijing 100005, China
2National Research Institute for Family Planing, WHO Collaboration Center for Research in Human Reproduction, 12 Da Hui Si, Beijing 100081, China
Correspondence: Lin Fang WANG(wanglf@ms.imicams.ac.cn)
Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry. In the testis seminiferous epithelium, VCY proteins were highly expressed in nuclei of germ cells. Using propidium iodide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainly localized in the nucleoli of COS7 cells. In addition, the colocalization for VCY and VCX-8r in COS7 cells was also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtained using yeast two-hybrid system. All the information above indicates that VCX/Y protein family might be involved in the regulation of ribosome assembly during spermatogenesis.
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