Volume 13, No 3, Jun 2003
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 13 Issue 3, June 2003: 195-202
ORIGINAL ARTICLES
The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine
Hsiao Chien TSUNG1,*, Zhong Wei DU**, Rong RUI***, Xiu Lan LI1, Lin Ping BAO1, Jun WU, Shi Min BAO2, Zhen YAO1
1Laboratory of Embryonic Stem Cell, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biologcal Sciences, Chinese Academy of Sciences, Shanghai 200031, China
2Shanghai Laboratory Animal Center, Chinese Academy of Sciences, Shanghai 200233, China
**Present address: Waisman center-University of Wisconsin T603, 1500 Highland Ave. Madison I 53705, USA. E-mail: zdu@wisc.edu
*** Who had joined and contributed to the project in the early stage while holding a postdoctoral scholarship during 1997-99, now at the College of Veterinary Medicine, Nanking Agricultural University, Jiangsu 210095, China. E-mail: rrui@njau.edu.cn
Correspondence: Hsiao Chien TSUNG(escg@sunm.shcnc.ac.cn)
As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.
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