Volume 13, No 2, Apr 2003
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 13 Issue 2, April 2003: 83-91
ORIGINAL ARTICLES
Cloning and characterization of human IC53-2, a novel CDK5 activator binding protein
Yi Hu XIE1, Xiang Huo HE1, Yun Tian TANG2, Jin Jun LI1, Zhi Mei PAN1, Wen Xin QIN1, Da Fang WAN 1*, Jian Ren GU1*
1State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China
2Peopele's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
Correspondence: Da Fang WAN, Jian Ren GU(nlorg@public.sta.net.cn)
We have identified IC53-2, a human homologue of the rat C53 gene from a human placenta cDNA library (GeneBank Accession No.AF217982). IC53-2 can bind to the CDK5 activator p35 by in vitro association assay. IC53-2 is mapped to human chromosome 17q21.31. The IC53-2 transcript is highly expressed in kidney, liver, skeletal muscle and placenta. It is abundantly expressed in SMMC-7721, C-33A, 3AO, A431 and MCF-7 cancer cell lines by RT-PCR assay. Stable transfection of IC53-2 cDNA into the hepatocellular carcinoma SMMC-7721 cell remarkably stimulates its growth in vitro. The above results indicate that IC53-2 is a novel human gene, which may be involved in the regulation of cell proliferation.
FULL TEXT | PDF
Browse 1690
