Volume 13, No 1, Feb 2003
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 13 Issue 1, February 2003: 21-28
ORIGINAL ARTICLES
Regulation of swelling-activated chloride channels in embryonic chick heart cells
Hua WEI1,2, Yan Ai MEI1, Jia Ting SUN1, Han Qing ZHOU1, Zhi Hong ZHANG1,*
1Department of Physiology and Biophysics, Liren Laboratory, School of Life Sciences, Fudan University, Shanghai 200433, China
2Fishery College, Shanghai Fisheries University, Shanghai 200090, China
Correspondence: Zhi Hong ZHANG(zhzhang@fudan.edu.cn)
Swelling-activated Cl
- currents, I
(Cl,swell), were measured during hyposmotic shock in white
Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I
(Cl,swell). Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKC, elicited the Cl
- current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I
(Cl,swell) in chelerythrine chloride(an inhibitor of PKC)-treated cells. Confocal microscopy experiments using FITC-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (CB) or cytochalasin D (CD) under isosmotic condition the disruption of the F-actin integrity was observed, and I
(Cl,swell) was not elicited. With combination treatment of CB with PMA, hyposmotic solution could not elicited I
(Cl,swell). The results suggested that the role of PTK, probably receptor tyrosine kinase, for regulation of I
(Cl,swell) appeared to be at upstream site related to the role of F-actin. Then PKC signal pathway was activated somehow and finally change in the polymerization state of cytoskeleton led to activate the swelling-activated Cl
- channels. These results demonstrate clearly that PTK, PKC and F-actin are important factors for regulation of I
(Cl,swell), in embryonic chick heart cells as compared with often controversial results reported in different cell types.
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