Volume 13, No 1, Feb 2003

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 13 Issue 1, February 2003: 59-68

ORIGINAL ARTICLES

Identification of ABA-responsive genes in rice shoots via cDNA macroarray

Fang LIN*, Shou Ling XU*, Wei Min NI, Zhao Qing CHU, Zhi Hong XU, Hong Wei XUE**

National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science (SIBS), Chinese Academy of Sciences; and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on ``Plant Molecular Physiology and Signal Transduction", 300 Fenglin Road, 200032 Shanghai, China Correspondence: These authors contribute equally to the paper.(hwxue@iris.sipp.ac.cn)

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In addition, some clones were further shown to be regulated by other plant growth regulators including auxin and brassinosteroid, which, however, indicated the complicated interactions of plant hormones. Possible signal transduction pathways involved in ABA were discussed.

FULL TEXT | PDF

Browse 2274