Volume 12, No 5-6, Dec 2002

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 12 Issue 5-6, December 2002: 401-405

ORIGINAL ARTICLES

Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping

Bang LIU, Geng Lin JIN, Shu Hong ZHAO, Mei YU, Tong An XIONG, Zhong Zhen PENG, Kui LI*

Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China Correspondence: Kui LI(lkxblghi@public.wh.hb.cn )

Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment combined with high chloroform Carnory's fixative solution from cells of the testes of domestic pigs. Comparison in the division index and length of pachytene bivalents with metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87-5.98) times longer than those of the latter. Comparative studies on chromomere maps of bivalents and mitotic chromosomal G-bands were conducted by using the chromosome 12 as a example. Sex vesicle and various shapes of synaptic sex chromosomes have been observed. Two-color PRimed IN Situ (PRINS) labeling has been conducted successfully on pachytene bivalents of pigs.

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