Volume 12, No 3-4, Sep 2002

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 12 Issue 3-4, September 2002: 229-233

ORIGINAL ARTICLES

Senescence-like changes induced by expression of p21^Waf1/Cip1 in NIH3T3 cell line

Xi CHEN¹, Wei ZHANG², Yun Fei GAO¹, Xiao Qin SU¹, Zhong He ZHAI^1,*

¹College of Life Sciences, Peking University, Beijing 100871, China
²The Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing 100875, China Correspondence:

P21^Waf1/Cip1 is a potent cyclin-dependent
kinase inhibitor. As a downstream mediator of p53, p21^Waf1/Cip1
involves in cell cycle arrest, differentiation and apoptosis. Previous studies
in human cells provided evidence for a link between p21^Waf1/Cip1 and
cellular senescence. While in murine cells, the role of p21^Waf1/Cip1
is indefinite. We explored this issue using NIH3T3 cells with inducible p21^Waf1/Cip1
expression. Induction of p21^Waf1/Cip1 triggered G1 growth arrest, and
NIH3T3-p21 cells exhibited morphologic features, such as enlarged and flattened
cellular shape, specific to the senescence phenotype. We also showed that p21^Waf1/Cip1-transduced
NIH3T3 cells expressed b -galactosidase activity at
pH 6.0, which is known to be a marker of senescence. Our results suggest that
p21^Waf1/Cip1 can also induce senescence-like changes in murine cells.

FULL TEXT | PDF

Browse 1868