Volume 12, No 1, Mar 2002
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 12 Issue 1, March 2002: 39-45
ORIGINAL ARTICLES
Identification of differentially expressed genes in human uterine leiomyomas using differential display
Bin LI1,*, Mei SUN1, *,#, Bin HE1,##, Jin YU2, You Duan ZHANG1, Yong Lian ZHANG1,**
1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Science, Chinese Academy of Sciences, 320, Yue-Yang Road, Shanghai, 200031, China
2OB/GYN Hospital , Shanghai (First) Medical University, 419, Fang-Xie Road, Shanghai, China
Correspondence:
In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS: Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named M1 in leiomyoma) were confirmed by Northern analysis.[2]Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19. [3] It was present ubiquitously in 13 tissues tested but in various levels and even in different size. [4] L1 was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPS19 overexpression migh be a universal signal in rapid cell growth tissues.
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