Volume 12, No 1, Mar 2002

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 12 Issue 1, March 2002: 63-68

ORIGINAL ARTICLES

The molecular characterization of maize B chromosome specific AFLPs

Zhong Xia QI, Hui ZENG, Xiu Lan LI, Cheng Bin CHEN, Wen QiN SONG, Rui Yang CHEN*

The College of Life Sciences, Nankai University, Tianjin 300071, China Correspondence:

The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGGGTTT conserved in plant chromosome telomeres. In addition, the sequence of M8-2D shared low homology to clones from maize chromosome 4 centromere as well. M8-2D were localized to B chromosome centromeric and telomeric regions.

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