Volume 11, No 4, Dec 2001
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 11 Issue 4, December 2001: 273-278
ORIGINAL ARTICLES
Auxin distribution and transport during embryogenesis and seed germination of Arabidopsis
NI Di An1,#, Ling Jian WANG 1, Chun Hong DING2,#,#, Zhi Hong XU1,3,*
1Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
2College of Bioscience, East China Normal University, Shanghai 200062, China
3College of Life Sciences, Peking University, Beijing 100871, China
#Present address: University of Potsdam, Institute of Biochemistry and Biology, AG Molecular-Biology,
Karl-Liebknecht-Str. 24-25, Hause 20, Golm14476, Germany
##Present address: Shanghai Experimental School of Elementary Education, Shanghai 201103, China
Correspondence:
Auxin distribution during embryogenesis and seed germination were studied with transgenic Arabidopsis plants expressing GUS gene driven by a synthetic DR5 promoter , an auxin responsive promoter. The results showed that GUS activity is higher in ends of hypophysis and cotyledon primordia of heart-, torpedo- and cotyledon-stage embryos, leaf tip area, lateral root primordia, root apex and cotyledon of young seedlings. And GUS accumulated in root apex of the seedlings grown on auxin transport inhibitor containing media. All these suggested that above-mentioned part of the organs and tissues have a higher level of auxin, and auxin polar transport inhibitor could cause the accumulation of auxin in root apex. And auxin transport inhibitor also resulted in aberration of Arabidopsis leaf pattern formation, root gravitropism and elongation.
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