Volume 11, No 4, Dec 2001

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 11 Issue 4, December 2001: 278-284

ORIGINAL ARTICLES

Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi

WANG Yu Mei¹, Jiang Bo WANG², Da LUO¹ , Jing Fen JIA^2,*

¹National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
²Department of Biology, Northwest University, Xi'an 710069, China Correspondence:

The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Plant regeneration was achieved from sterile calluses derived from hairy roots, which occurred at or near the infection sites. The hairy root regenerants were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T-DNA had been intergrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi.

FULL TEXT | PDF

Browse 1639