Volume 11, No 4, Dec 2001

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 11 Issue 4, December 2001: 285-291

ORIGINAL ARTICLES

Overexpression of a novel gene, Cms1, can rescue the growth arrest of a Saccharomyces cerevisiae mcm10 suppressor

WANG Ji Wu, Jia Rui WU*

State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Research Center of Life Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China Correspondence:

MCM10 protein is an essential replication factor involved in the initiation of DNA replication. A mcm10 mutant (mcm10-1) of budding yeast shows a growth arrest at 37oC. In the present work, we have isolated a mcm10-1 suppressor strain, which grows at 37oC. Interestingly, this mcm10-1 suppressor undergoes cell cycle arrest at 14oC. A novel gene, YLR003c, is identified by high-copy complementation of this suppressor. We called it as Cms1 (Complementation of Mcm10 Suppressor). Furthermore, the experiments of transformation show that cells of mcm10-1 suppressor with high-copy plasmid but not low-copy plasmid grow at 14oC, indicating that overexpression of Cms1 can rescue the growth arrest of this mcm10 suppressor at non-permissive temperature. These results suggest that CMS1 protein may functionally interact with MCM10 protein and play a role in the regulation of DNA replication and cell cycle control.

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