Volume 11, No 3, Sep 2001

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 11 Issue 3, September 2001: 181-186

REVIEWS

Gene silencing: Double-stranded RNA mediated mRNA degradation and gene inactivation

TANG Wei^1,*, Xiao Yan LUO², Vanessa SANMUELS¹

¹North Carolina State University, Forest Biotechnology Group, Raleigh, NC 27695, USA
²University of North Carolina, Department of Cell and Developmental Biology, Chapel Hill, NC 27599, USA Correspondence:

The recent development of gene transfer approaches in plants and animals has revealed that transgene can undergo silencing after integration in the genome. Host genes can also be silenced as a consequence of the presence of a homologous transgene. More and more investigations have demonstrated that double-stranded RNA can silence genes by triggering degradation of homologous RNA in the cytoplasm and by directing methylation of homologous nuclear DNA sequences. Analyses of Arabidopsis mutants and plant viral suppressors of silencing are unraveling RNA-silencing mechanisms and are assessing the role of methylation in transcriptional and posttranscriptional gene silencing. This review will focus on double-stranded RNA mediated mRNA degradation and gene inactivation in plants.

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