Volume 11, No 3, Sep 2001

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 11 Issue 3, September 2001: 187-193

ORIGINAL ARTICLES

Isolation of a choline monooxygenase cDNA clone from Amaranthus tricolor and its expressions under stress conditions

MENG Yu Ling¹, Yu Mei WANG², Da Bing ZHANG³, Naosuke NII^1,*

¹Faculty of Agriculture, Meijo University, Tempaku-ku, Nagoya, Aichi. 468-8502 Japan
²Yingdong College of Biotechnology, Shaoguan University, Datang Road, Shaoguan 512005, China
³Agro-Biotech Center of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of genetic breeding, 2901 Beidi Road, Shanghai 201106, China Correspondence:

Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine betaine[1]. Two enzymes are involved in the pathway, choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). A full length CMO cDNA (1,643bp) was cloned from Amaranthus tricolor. The open reading frame encoded a 442-amino acid polypeptide, which showed 69% identity with CMOs in Spinacia oleracea L. and Beta vulgaris L. DNA gel blot analysis indicated the presence of one copy of CMO gene in the A. tricolor genome. The expressions of CMO and BADH proteins in A.tricolor leaves significantly increased under salinization, drought and heat stress (42oC), as determined by immunoblot analysis, but did not respond to cold stress (4oC), or exogenous ABA application. The increase of GB content in leaves was parallel to CMO and BADH contents.

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