Volume 11, No 3, Sep 2001
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 11 Issue 3, September 2001: 203-208
ORIGINAL ARTICLES
A mimotope of Pre-S2 region of surface antigen of viral hepatitis B screened by phage display
WU Yu Zhang1, Ying WAN1, Dao Guo LI2, Yan TANG1, Wei ZHOU1
1Institute of Immunology, PLA, The Third Medical University, Chongqing 400038, China
2Medical Center, General Hospital of Weifang, Weifang 26100, China
Correspondence:
To acquire the phage-displayed mimotopes which mimic the specificity of hepatitis B virus surface antigen (HBsAg), a random peptide library expressing linear peptide with 12 amino acids in length were used to screen with the serum from a hepatitis B virus infected patient in the recovery phase. After 3 rounds of biopanning, the positive phages were confirmed by competitive ELISA using HBsAg/P33. Two phagotopes were identified and one of them was confirmed as mimotope by competition experiment. Based on the mimotpe, a multiple antigenic peptide with four branches was synthesized by solid phase peptide synthesis. The antiginicity and specificity of the synthesized antigen was tested in BALB/c mice compared with the native epitope-based antigen. The results showed that the mimotope-based antigen could evoke higher titer of antibodies with the same specificity of the epitope-based antigen. Those findings indicate mimotopes can be used in antigen and vaccine design.
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