Volume 11, No 3, Sep 2001

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 11 Issue 3, September 2001: 217-222

ORIGINAL ARTICLES

TNF receptor-associated factor-2 binding site is involved in TNFR75-dependent enhancement of TNFR55-induced cell death

LU Fang, Jia FANG, Chang Qing CHEN*

Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences, Shanghai 200233, China Correspondence:

TNF recepter-55
is the main mediator of TNF-induced apoptosis. TNF receptor-75-dependent
induction or enhancement of cytotoxicity has been explained by intracellular
signaling, ``ligand passing", or induction of endogenous TNF. To study the
function of human TNF receptor-75 (hTR75) and the interaction between human TNF
receptor-55 (hTR55) and hTR75 in hTNFa-induced
cytotoxicity, HEp-2 cells were transfected with bicistronic expression vector of
hTR75 and its deletion mutants genes. hTNFa-induced
cytotoxicity was determined by crystal violet colorimetric method. The
expression of hTR75 and its deletion mutants in HEp-2 cells was demonstrated by
RT-PCR and indirect ELISA. We found that the overexpressed hTR75 could
significantly increase the susceptibility of HEp-2 cells to hTNFa
which especially required TRAF2 binding site. hTR75
could not only mediate partial hTNFa-induced
cytotoxicity independently but also fulfill an accessory role in enhancing or
synergizing hTR55-mediated cytotoxicity.

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