Volume 11, No 2, Jun 2001
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 11 Issue 2, June 2001: 124-135
ORIGINAL ARTICLES
Altered expression of nuclear matrix proteins in etoposide induced apoptosis in HL-60 cells
JIN Mei Lin1,*, Ping ZHANG3, Ming Xiao DING2, Jing Ping YUN3, Pei Fang CHEN3, Yeuk Hon CHEN3, Yin Qing CHEW3
1College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2College of Life Sciences, Beijing University, Beijing 100871, China
3Chinese University of Hong Kong, Hong Kong, China
Correspondence:
The events of cell death and the expression of nuclear matrix protein (NMP) have been investigated in a promyelocytic leukemic cell line HL-60 induced with etoposide. By means of TUNEL assay, the nuclei displayed a characteristic morphology change, and the amount of apoptotic cells increased early and reached maximun about 39% after treatment with etoposide for 2 h. Nucleosomal DNA fragmentation was observed after treatment for 4 h. The morphological change of HL-60 cells, thus, occurred earlier than the appearance of DNA ladder. Total nuclear matrix proteins were analyzed by 2-dimensional gel electrophoresis. Differential expression of 59 nuclear matrix proteins was found in 4 h etoposide treated cells. Western blotting was then performed on three nuclear matrix acssociated proteins, PML, HSC70 and NuMA. The expression of the suppressor PML protein and heat shock protein HSC70 were significantly upregulated after etoposide treatment, while NuMA, a nuclear mitotic apparatus protein, was down regulated. These results demonstrate that significant biochemical alterations in nuclear matrix proteins take place during the apoptotic process.
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