Volume 11, No 1, Mar 2001

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 11 Issue 1, March 2001: 37-43

ORIGINAL ARTICLES

Hydrogen peroxide-induced changes in intracellular pH of guard cells precede stomatal closure

ZHANG Xiao^1,2, Fa Cai DONG¹, Jun Feng GAO², Chun Peng SONG^1,*

¹Department of Biology, Henan University, Kaifeng 475001, China
²College of Life Sciences, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China Correspondence:

Epidermal bioassay demonstrated that benzylamine, a membrane-permeable weak base, can mimick hydrogen peroxide (H₂O₂) to induce stomatal closure, and butyric acid, a membrane-permeable weak acid, can partly abolish the H₂O₂-induced stomatal closure. Confocal pH mapping with the probe 5-(and-6)-carboxy seminaphthorhodafluor-1-acetoxymethylester (SNARF-1-AM) revealed that H₂O₂ leads to rapid changes in cytoplasmic and vacuolar pH in guard cells of Vicia faba L, i. e. alkalinization of cytoplasmic areas occur red in parallel with a decrease of the vacuolar pH, and that butyric acid pretreatment can abolish alkalinization of cytoplasmic areas and acidification of vacuolar areas of guard cells challenged with H₂O₂. These results imply that the alkalinization of cytoplasm via efflux of cytosol protons into the vacuole in guard cells challenged with H₂O₂ is important at an early stage in the signal cascade leading to stomatal closure.

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