Volume 11, No 1, Mar 2001
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 11 Issue 1, March 2001: 68-73
ORIGINAL ARTICLES
Structural components of the nuclear body in nuclei of Allium cepa cells
TAO Wei1, Chang Hui YAN1, Tao CAI1, Shui HAO2, Zhong He ZHAI1,*
1College of Life Sciences, Peking University, Beijing 100871, China
2Institute of Genetics and Cytology, Northeast Normal University, Changchun Jilin 130024, China
Correspondence:
Nuclear bodies have long been noted in interphase nuclei of plant cells, but their structural component, origin and function are still unclear by now. The present work showed in onion cells the nuclear bodies appeared as a spherical structure about 0.3 to 0.8 μm in diameter. They possibly were formed in nucleolus and subsequently released, and entered into nucleoplasm. Observation through cytochemical staining method at the ultrastructural level confirmed that nuclear bodies consisted of ribonucleoproteins (RNPs) and silver-stainable proteins. Immunocytochemical results revealed that nuclear bodies contained no DNA and ribosomal gene transcription factor (UBF). Based on these data, we suggested that nuclear bodies are not related to the ribosome or other gene transcription activities, instead they may act as subnuclear structures for RNPs transport from nucleolus to cytoplasm, and may also be involved in splicing of pre-mRNAs.
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