Volume 10, No 3, Sep 2000

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 10 Issue 3, September 2000: 233-242

ORIGINAL ARTICLES

Mitochondria, supernatant, apoptosis, egg extract, nuclei, DNA cleavage.

CHANG Yun Chao, Yong Hua XU*

Shanghai Institute of Cell Biology, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China Correspondence:

Apoptosis plays an important role in embryonic development, tissue remodeling, immune regulation and tumor regression. Two groups of molecules (Bcl-2 family and "Death factor" family) are involved in regulating apoptosis. In order to know about the effect of Bcl-2 on apoptosis induced by Fas, a typical member of "Death factor" family, the transfection experiments with expression vectors pcDNA3- fl and pcDNA3- bcl-2 were performed in BEL-7404 cells, a human hepatocellular carcinoma cell line which expresses endogenous Fas, but not FasL and Bcl-2. The data showed that the expression of FasL in pcDNA3- fl transfected hepatoma cells obviously induced the apoptosis of the cells. However, the overexpression of Bcl-2 in pcDNA3- bcl-2 transfected 7404/b-16 cells counteracted pcDNA3- fl transient transfection mediated apoptosis. Further study by co-transfection experiments indicated that Bid but not Bax (both were pro-apoptotic proteins of Bcl-2 family) blocked the inhibitory effect of Bcl-2 on Fas-mediated apoptosis. These results suggested that Fas-mediated apoptosis in human hepatoma cells is possibly regulated by Bcl-2 family proteins via mitochondria pathway.

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