Volume 10, No 2, Jun 2000
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 10 Issue 2, June 2000: 139-149
ORIGINAL ARTICLES
Activation of phospholipase D activity in transforming growth factor-beta-induced cell growth inhibition
ZHOU Bing Hong, Jun Song CHEN, Ming Qiang CHAI, Sheng ZHAO, Jun LIANG, He Hua CHEN, Jian Guo SONG*
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
Correspondence:
Cells regulate phospholipase D (PLD) activity in response to numerous extracellular signals. Here, we investigated the involvement of PLD activity in transforming growth factor-
b (TGF-
b1)-mediated growth inhibition of pithelial cells. TGF-
b inhibits the growth of MDCK, Mv1Lu, and A-549 cells. In the presence of 0.4 % butanol, TGF-
b induces an increase in the formation of phosphatidylbutanol, a unique product catalyzed by PLD. TGF-
b also induces an increase in phosphatidic acid (PA) level in A-549 and MDCK cells. TGF-
b induces an increase in the levels of DAG labeled with [
3H]-myristic acid in A-549 and MDCK cells but not in Mv1Lu cells. No increase of DAG was observed in cells prelabeled with [
3H]-arachidonic acid. The data presented suggest that PLD activation is involved in the TGF-
b-induced cell growth inhibition.
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