Volume 10, No 2, Jun 2000

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 10 Issue 2, June 2000: 151-160

ORIGINAL ARTICLES

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

HE Yu Ke*, Wan Xin XUE, Yu Dong SUN, Xu Hong YU, Ping Lin LIU

National Laboratory of Plant Molecular Genetics, Shanghai
Institute of Plant Physiology, Chinese Academy of Sciences,
Shanghai 200032, China Correspondence:

The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were self-crossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization. Two lines, D1232 and D1653, showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leafy head. Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage, and the cuttings from head leaves had more roots than rosette leaves at heading stage. It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin. These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant organs and for the breeding of new variety with rapid growth trait.

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